RANKL/OPG in primary cultures of osteoblasts from post-menopausal women. Differences between osteoporotic hip fractures and osteoarthritis |
| |
| Authors: | Mercè Giner Ma José Rios Ma José Montoya Ma Angeles Vázquez Latifa Naji Ramón Pérez-Cano |
| |
| Institution: | 1. Columbia University, College of Physicians and Surgeons, Division of Rheumatology, United States;2. Royal National Hospital for Rheumatic Diseases, Rheumatology Department, Bath, UK;3. Cambridge University Hospitals NHS Foundation Trust, Rheumatology Department, Department of Rheumatology, Addenbrooke''s Hospital, Cambridge, UK;4. Department of Rheumatology, St. Vincent''s University Hospital and Conway Institute, University College Dublin, Dublin, Ireland;5. University Hospital Kerry, Kerry, Ireland;1. Departamento de Fisiología de la Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México, D.F., México;2. Nutrition and Genomics’ Laboratory, Jean Mayer United States Department of Agriculture Human Nutrition Research Center on Aging at Tufts University, Boston, MA, USA;1. Center for Translational Medicine, The First Affiliated Hospital of Xi''an Jiaotong University, Xi''an 710061, China;2. The Department of Joint Surgery, Knee Word, Honghui Hospital, Xian Jiaotong University, Xi''an 710054, China;3. Department of Anesthesiology, Honghui Hospital, Xi’an Jiaotong University, Xi''an 710054, China |
| |
| Abstract: | The OPG/RANKL/RANK system is important in the balance between bone formation and resorption.We used primary human osteoblasts (hOBs) cells to examine the impact of 17-β-estradiol (E2) or/and 1,25-dihydroxyvitamin D (1,25D) in OPG/RANKL system in 28 post-menopausal (PM) women; (a) with hip fracture (OP) or (b) with osteoarthritis (OA). The hOB from OP patients proliferated slower during the first stage, than the OA women (31.5 ± 2.6 and 21.4 ± 1.3 days, respectively, p < 0.05). The OP group secreted significantly higher OPG protein levels than the OA women (10.1 ± 2.6 and 4.4 ± 0.8 pmol/L, respectively, p < 0.05). The 1,25D and 1,25D+E2 induce an increase in RANKL and RANKL/OPG mRNA expression in OP patients above 200% (p < 0.05).HOBs from the osteoporotic hip initially proliferate slower but after reaching the first cellular confluence, the proliferation rate is equal in both groups. Furthermore, hOBs from hips with OP present a higher protein secretion of OPG, and higher RANKL and RANKL/OPG expression ratio in response to 1,25D and 1,25D+E2, than hOBs from OA women. All this could suggest that the greater bone loss that characterizes OP patients can be mediated due to differences in the secretion and expression of the RANKL/OPG system in response to different stimuli. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
