灵芝孢子粉中脂溶性成分分析方法的建立

运用高效液相建立灵芝孢子粉中脂溶性成分的分析测定方法。通过色谱柱、洗脱条件、ELSD参数的优化,建立了12种脂溶性成分的测定方法,结果表明,该方法简单、准确、稳定,可以实现孢子粉中甘油三酯、脂肪酸、甾醇三类脂溶性成分的同时提取、分析;破壁孢子粉中脂溶性成分为301.49-397.37mg/g,孢子油产品中脂溶性成分的含量为626.00-713.07mg/g,远高于三萜含量;1,2-二油酸-3-棕榈酸甘油酯、甘油三油酸酯、1,2-二油酸-3-亚油酸甘油酯是主要的脂溶性成分,脂肪酸以不饱和脂肪酸亚油酸及油酸为主,甾醇中麦角甾醇含量最高。研究结果明确了灵芝孢子粉中脂溶性成分的物质基础,为深入研究其活性成分、全面评价孢子粉质量提供了依据。

Determination of liposoluble components in Ganoderma lingzhi spore powder

A high-performance liquid phase was established to determine the content of liposoluble components in Ganoderma lingzhi spore powder. The determination of 12 liposoluble components was carried out by optimizing the column, elution conditions and ELSD parameters. The method proved to be simple, accurate, stable, and appropriate to the simultaneous quantitative detection of triglycerides, fatty acids and sterols in G. lingzhi spore powder. The analysis of multiple samples showed that the liposoluble components were 301.49-397.37mg/g in the broken spore powder, and 626.00-713.07mg/g in the spore oil product, being higher than the content of triterpenes. 1,2-Olein-3-palmitin, triolein and 1,2-Olein-3-linolein were the main liposoluble components, and the fatty acids were mainly unsaturated fatty acids including linoleic acid and oleic acid. The content of ergosterol was the highest in sterols. The research clarified the liposoluble components in G. lingzhi spore powder, and provided reference for further investigation of active ingredients and comprehensive evaluation of quality control of G. lingzhi spore powder.