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Surface plasmon resonance-based detection of ladder-shaped polyethers by inhibition detection method
Authors:Ryota Mouri  Tohru Oishi  Kohei Torikai  Satoru Ujihara  Nobuaki Matsumori  Michio Murata  Yasukatsu Oshima
Institution:1. Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan;2. Graduate School of Life Sciences, Tohoku University, Tsutsumidori-Amemiya, Aoba-ku, Sendai 981-8555, Japan
Abstract:Ladder-shaped polyether (LSP) compounds represented by brevetoxins and ciguatoxins were largely discovered in association with seafood poisoning. Thus, a quick quantification method for LSPs is potentially important. We examined a surface plasmon resonance method using desulfated-yessotoxin (dsYTX) immobilized on a sensor chip and phosphodiesterase PDEII in a inhibition detection mode. Yessotoxin, brevetoxin B and synthetic LSP derivatives showed clear inhibition against PDEII binding to the immobilized dsYTX, by which their half inhibitory concentrations were successfully estimated. This inhibition method appeared to be superior in specificity to direct binding assays where binding proteins to LSP was immobilized on a sensor chip.
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