Biodiesel production from sunflower,soybean, and waste cooking oils by transesterification using lipase immobilized onto a novel microporous polymer |
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| Authors: | Nadir Dizge Coskun Aydiner Derya Y. Imer Mahmut Bayramoglu Aziz Tanriseven Bülent Keskinler |
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| Affiliation: | 1. Gebze Institute of Technology, Department of Environmental Engineering, Gebze 41400, Turkey;2. Gebze Institute of Technology, Department of Chemical Engineering, Gebze 41400, Turkey;3. Gebze Institute of Technology, Department of Biochemistry, Gebze 41400, Turkey;1. Centre for Energy Sciences, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia;2. Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia;3. Department of Mechanical Engineering, Medan State Polytechnic, 20155 Medan, Indonesia;4. Department of Mechanical Engineering, Universiti Tenaga Nasional, 43000 Kajang, Selangor, Malaysia;5. Generation & Environment Unit, TNB Research, No. 1 Lorong Ayer Itam, 43000 Kajang, Selangor, Malaysia;1. Bart?n University, Department of Environmental Engineering, 74100 Center, Bart?n, Turkey;2. Istanbul Technical University, Ekrem Elginkan Private High School, 34846 Maslak, Istanbul, Turkey;3. Gebze Institute of Technology, Department of Environmental Engineering, 41400 Gebze, Kocaeli, Turkey;4. National Research Center on Membrane Technology, 80626 Maslak, Istanbul, Turkey;5. Istanbul Technical University, Department of Environmental Engineering, 80626 Maslak, Istanbul, Turkey;1. Istanbul University, Faculty of Engineering, Department of Environmental Engineering, 34320 Istanbul, Turkey;2. Gebze Institute of Technology, Faculty of Engineering, Department of Environmental Engineering, 41400 Kocaeli, Turkey;1. Laboratoire d’Etude et de Développement des Techniques de Traitement et d’Epuration des Eaux et de Gestion Environnementale (LEDTEGE), Ecole Normale Supérieure de Kouba, BP 92, Vieux-Kouba, 16308 Alger, Algeria;2. Département de Physique, Ecole Normale Supérieure de Laghouat, BP 4033, RP Rue des martyrs, Laghouat, Algeria;3. Department of Environmental Engineering, Mersin University, Mersin, 33343, Turkey;4. Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, BP 92 Vieux-Kouba, 16308, Alger, Algeria;5. Département de Biologie, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre, Université de Ghardaïa, BP 455, Ghardaïa 47000, Algeria;6. Department of Environmental Engineering, Gebze Technical University, Kocaeli, 41400, Turkey |
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| Abstract: | This study aims at carrying out lipase-catalyzed synthesis of fatty acid methyl esters (biodiesel) from various vegetable oils using lipase immobilized onto a novel microporous polymeric matrix (MPPM) as a low-cost biocatalyst. The research is focused on three aspects of the process: (a) MPPM synthesis (monolithic, bead, and powder forms), (b) microporous polymeric biocatalyst (MPPB) preparation by immobilization of lipase onto MPPM, and (c) biodiesel production by MPPB. Experimental planning of each step of the study was separately carried out in accordance with design of experiment (DoE) based on Taguchi methodology.Microporous polymeric matrix (MPPM) containing aldehyde functional group was synthesized by polyHIPE technique using styrene, divinylbenzene, and polyglutaraldehyde. Thermomyces lanuginosus lipase was covalently attached onto MPPM with 80%, 85%, and 89% immobilization efficiencies using bead, powder, and monolithic forms, respectively. Immobilized enzymes were successfully used for the production of biodiesel using sunflower, soybean, and waste cooking oils. It was shown that immobilized enzymes retain their activities during 10 repeated batch reactions at 25 °C, each lasting 24 h. Since the developed novel method is simple yet effective, it could have a potential to be used industrially for the production of chemicals requiring immobilized lipases. |
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