Lithospermic acid derivatives from Lithospermum erythrorhizon increased expression of serine palmitoyltransferase in human HaCaT cells |
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Authors: | Phuong Thien Thuong Keon Wook Kang Jeong Kee Kim Dae Bang Seo Sang Jun Lee Sung Han Kim Won Keun Oh |
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Affiliation: | 1. College of Pharmacy, Chosun University, 375 Seosuk-dong, Dong-gu, Gwangju 501-759, Republic of Korea;2. AmorePacific Corporation, 314-1 Bora-ri, Yongin, Gyeonggi-do 449-729, Republic of Korea;3. Nutrex Technology, 218 Yangjae-dong, Seocho-gu, Seoul 137-893, Republic of Korea |
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Abstract: | A MeOH extract of the dry root of Lithospermum erythrorhizon showed strong increasing effect on serine palmitoyltransferase (SPT) in normal human keratinocyte cells (HaCaT cells). Bioassay-guided separation on this extract using repeated chromatography resulted in the isolation of lithospermic acid (1) and two derivative esters, 9″-methyl lithospermate (2) and 9′-methyl lithospermate (3). Compounds 1–3 significantly increased SPT expressions in the relative quantity (%) of SPT1 mRNA as well as SPT2 mRNA. These constituents also raised the level of SPT protein in HaCaT cells in a dose-dependent manner, with the increased level of SPT protein in HaCaT cells of 55%, 23%, and 81% at the concentration of 100 μg/ml, respectively. This finding suggests that lithospermic acid and its derivatives from L. erythrorhizon might improve the permeability barrier by stimulating the protein level of SPT. |
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