Comparative hydrolysis and fermentation of sugarcane and agave bagasse |
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| Authors: | JM Hernández-Salas MS Villa-Ramírez JS Veloz-Rendón KN Rivera-Hernández RA González-César MA Plascencia-Espinosa SR Trejo-Estrada |
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| Institution: | 1. Departamento de Ingeniería Química, CUCEI-Universidad de Guadalajara, Blvd. M. García Barragán 1451, C.P. 44430 Guadalajara, Jalisco, Mexico;2. División de Procesos Industriales, Universidad Tecnológica de Jalisco, Luis J. Jiménez 577-1° de Mayo, C.P. 44979, Guadalajara, Jalisco, Mexico;1. Department of Chemical Engineering, Universidad Autónoma de Nayarit, Tepic, Mexico;2. Department of Chemical Engineering and Materials Science, DOE Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, MI, United States;3. Biorefinery Group, Food Research Department, School of Chemistry, Autonomous University of Coahuila, Saltillo, Coahuila, Mexico;4. Department of Chemical Engineering and Conn Center for Renewable Energy Research, University of Louisville, Louisville, KY, United States;5. Joint BioEnergy Institute, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Emeryville, CA, United States;6. Sandia National Laboratories, Biological and Engineering Sciences Center, Livermore, CA, United States;1. Department of Chemical Engineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India;2. Netaji Subhas Institute of Technology, Division of Biotechnology, New Delhi 110078, India;1. Chinese Academy of Sciences, Biomass Group, Key Laboratory of Tropical Plant Resource and Sustainable Use, Xishuangbanna Tropical Botanical Garden, 88 Xuefulu, Kunming, Yunnan Province 650223, China;2. University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China;1. Central Queensland University, School of Medical and Applied Sciences, Rockhampton 4702, Queensland, Australia;2. NSW Department of Primary Industries, 1243 Bruxner Highway, Wollongbar, NSW 2477, Australia |
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| Abstract: | Sugarcane and agave bagasse samples were hydrolyzed with either mineral acids (HCl), commercial glucanases or a combined treatment consisting of alkaline delignification followed by enzymatic hydrolysis. Acid hydrolysis of sugar cane bagasse yielded a higher level of reducing sugars (37.21% for depithed bagasse and 35.37% for pith bagasse), when compared to metzal or metzontete (agave pinecone and leaves, 5.02% and 9.91%, respectively). An optimized enzyme formulation was used to process sugar cane bagasse, which contained Celluclast, Novozyme and Viscozyme L. From alkaline–enzymatic hydrolysis of sugarcane bagasse samples, a reduced level of reducing sugar yield was obtained (11–20%) compared to agave bagasse (12–58%). Selected hydrolyzates were fermented with a non-recombinant strain of Saccharomyces cerevisiae. Maximum alcohol yield by fermentation (32.6%) was obtained from the hydrolyzate of sugarcane depithed bagasse. Hydrolyzed agave waste residues provide an increased glucose decreased xylose product useful for biotechnological conversion. |
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