Statistical media optimization for growth and PHB production from methanol by a methylotrophic bacterium |
| |
| Authors: | Zahra B Mokhtari-Hosseini Ebrahim Vasheghani-Farahani Ali Heidarzadeh-Vazifekhoran S Abbas Shojaosadati Ramin Karimzadeh Kianoush Khosravi Darani |
| |
| Institution: | 1. Biotechnology Group, Faculty of Engineering, Tarbiat Modares University, P.O. Box 14115-143, Tehran, Iran;2. National Nutrition and Food Technology Research Institute, Shahid Beheshti Medical University, P.O. Box 19395-4741, Tehran, Iran;1. Department of Post Harvest Process and Food Engineering, G.B. Pant University of Agriculture and Technology, Pantnagar 263 145, India;2. Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow Campus, Lucknow 226010, India;3. Bioenergy and Energy Planning Research Group, Ecole Polytechnique Federale de Lausanne (EPFL), Lausanne, Switzerland;4. Microbial Processes and Technology Division, CSIR-National Institute of Interdisciplinary Science and Technology (CSIR-NIIST), Trivandrum 695 019, India;1. Biotechnology Research Laboratory, Faculty of Chemical Engineering, Babol Noshirvani University of Technology, Babol, Iran;2. Department of Environmental Science, Faculty of Natural Resources, Tarbiat Modares University (TMU), Nour, Iran |
| |
| Abstract: | Media components were optimized by statistical design for cell growth and PHB production of Methylobacterium extorquens DSMZ 1340. Four important components of growth media were optimized by central composite design. The growth increased from an OD = 1.35 for Choi medium as control to an OD = 2.15 for optimal medium. Then media components for PHB production were optimized. Optimization of five important factors was conducted by response surface method. The optimal composition of PHB production medium was found to be at 7.8 (g/L) Na2HPO4 · 12H2O, and surprisingly at zero concentration of (NH4)2SO4, KH2PO4, MgSO4 and MnSO4. The PHB production was found to be 2.95 (g/L) at this medium. RSM results indicated that a deficiency of nitrogen and magnesium is crucial for PHB accumulation in this microorganism. Also, PHB production was carried out in a 5 L fermentor at the optimum condition which resulted in 9.5 g/L PHB and 15.4 g/L cell dry weight with 62.3% polymer content. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
