Fermentative production of succinic acid from straw hydrolysate by Actinobacillus succinogenes |
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| Authors: | Pu Zheng Jin-Jun Dong Zhi-Hao Sun Ye Ni Lin Fang |
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| Affiliation: | 1. Departamento de Ingeniería Química, CUCEI-Universidad de Guadalajara, Blvd. M. García Barragán 1421, C.P. 44430 Guadalajara, Jalisco, Mexico;2. Unidad de Tecnología Agroalimentaria, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. (CIATEJ), A.C. Av. Normalistas 800, Colinas de la Normal, C.P. 44270 Guadalajara, Jalisco, Mexico;3. Departamento de Madera Celulosa y Papel, Universidad de Guadalajara, Km. 15.5 carretera Guadalajara-Nogales, C.P. 45110 Zapopan, Jalisco, Mexico;1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Puzhu South Road 30#, Nanjing 211816, PR China;2. Department of Biology, College of Natural and Computational Science, Mizan-Tepi University, PO Box 121, Tepi, Ethiopia;3. Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing 211800, PR China;1. Guangxi Key Laboratory of Subtropical Bio-resource Conservation and Utilization, College of Life Science and Technology, Guangxi University, Nanning, Guangxi 530005, China;2. National Non-grain Bio-energy Engineering Research Center, Guangxi Academy of Sciences, Nanning, Guangxi 530007, China;3. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China;1. DSM Biotechnology Center, Alexander Fleminglaan 1, 2613 AX Delft, The Netherlands;2. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands |
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| Abstract: | In this work, straw hydrolysates were used to produce succinic acid by Actinobacillus succinogenes CGMCC1593 for the first time. Results indicated that both glucose and xylose in the straw hydrolysates were utilized in succinic acid production, and the hydrolysates of corn straw was better than that of rice or wheat straw in anaerobic fermentation of succinic acid. However, cell growth and succinic acid production were inhibited when the initial concentration of sugar, which was from corn straw hydrolysate (CSH), was higher than 60 g l?1. In batch fermentation, 45.5 g l?1 succinic acid concentration and 80.7% yield were attained after 48 h incubation with 58 g l?1 of initial sugar from corn straw hydrolysate in a 5-l stirred bioreactor. While in fed-batch fermentation, concentration of succinic acid achieved 53.2 g l?1 at a rate of 1.21 g l?1 h?1 after 44 h of fermentation. Our work suggested that corn straw could be utilized for the economical production of succinic acid by A. succinogenes. |
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