Ecological significance and some biotechnological application of an organic solvent stable alkaline serine protease from Bacillus subtilis strain DM-04 |
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| Authors: | Sudhir K. Rai Ashis K. Mukherjee |
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| Affiliation: | 1. Fachgebiet Industrielle Biokatalyse, Technische Universität München, Lichtenbergstr. 4, 85748 Garching, Germany;2. Institute of Technical Microbiology, Hamburg University of Technology (TUHH), Kasernenstr. 12, 21073 Hamburg, Germany;1. Department of Microbiology, BMS Block, Panjab University, Chandigarh, India;2. Department of Industrial Microbiology, Guru Nanak Khalsa College, Yamunanagar, Haryana, India;1. Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran;2. Department of Biology, University of Sistan and Baluchestan, Zahedan, Iran;1. Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia;2. Laboratory of Natural Products Chemistry and Biomolecules (LNPCB), University of Blida 1, Road of Soumaâ, PO Box 270, 09000 Blida, Algeria;3. National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA) 11, Bd Amirouche PO Box 67, Bou Ismaïl, 42415 Tipaza, Algeria;1. Laboratory of Natural Products Chemistry and Biomolecules (LNPC-BioM), Faculty of Sciences, University of Blida 1, Road of Soumaâ, PO Box 270, 09000 Blida, Algeria;2. Laboratoire de Biologie des Systèmes Microbiens (LBSM), École Normale Supérieure (ENS) de Kouba, PO Box 92 Vieux-Kouba, 16308, Alger, Algeria;3. Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia;4. Laboratory of Cellular and Molecular Biology, Microbiology Team, Faculty of Biological Sciences, University of Sciences and Technology of Houari Boumediene (USTHB), PO Box 32, El Alia, Bab Ezzouar, 16111 Algiers, Algeria;5. Laboratory of Molecular and Cellular Screening Processes (LMCSP), Genomics and Bioinformatics Group, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia;6. National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11 Bd Amirouche PO Box 67, Bou Ismaïl, 42415, Tipaza, Algeria |
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| Abstract: | An organic solvent stable, alkaline serine protease (Bsubap-I) with molecular mass of 33.1 kDa, purified from Bacillus subtilis DM-04 showed optimum activity at temperature and pH range of 37–45 °C and 10.0–10.5, respectively. The enzyme activity of Bsubap-I was significantly enhanced in presence of Fe2+. The thermal resistance and stability and of Bsubap-I in presence of surfactants, detergents, and organic solvents, and its dehairing activity supported its candidature for application in laundry detergent formulations, ultrafiltration membrane cleaning, peptide synthesis and in leather industry. The broad substrate specificity and differential antibacterial property of Bsubap-I suggested the natural ecological role of this enzyme for the producing bacterium. |
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