Isolation and characterization of a higher-copy-number mutant of plasmid R6K |
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Authors: | J Ne?vera J Hochmannová |
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Institution: | (1) Department of Molecular Biology and Genetics, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20 Prague 4 |
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Abstract: | A stable copy-number mutant (pNH601) of plasmid R6K was isolated by selection for increased resistance to ampicillin determined
by this plasmid. The size of the mutant plasmid was found to be unchanged (26 Mg/mol) but it is present in 27 copies of pNH601
perE. coli K-12 chromosome which represents a two-fold increase of R6K copy number value. The following genetic properties of pNH601
are reported and compared with those of R6K: conjugative transfer, fertility inhibition of plasmids belonging to other incompatibility
groups, incompatibility with plasmid R485 under both non-selective and selective conditions and the integrative suppression
of thednaA ts mutation. The mutant plasmid pNH601 was found to be different from the original R6K in most of these properties. |
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