RNA synthesis in cultured human placenta |
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| Authors: | H. Lorberboum D. Weinstein H. Galski N. de Groot S. Segal J. Ilan R. Folman A. A. Hochberg |
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| Affiliation: | (1) Department of Biological Chemistry, The Hebrew University of Jerusalem, Institute of Life Sciences, Jerusalem, Israël;(2) Department of Obstetrics and Gynecology, Hadassah-Hebrew University Medical School, Ein Karem, Jerusalem, Israël;(3) The Rockefeller Foundation, Rockefeller University, New York, NY, USA;(4) Department of Reproductive Biology, Case Western Reserve University, School of Medicine, Cleveland, OH, USA |
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| Abstract: | The in vitro synthesis of RNA in the human placental tissue, incubated in organ culture, was investigated. We followed the synthesis of the poly A(-) and poly A(+) RNA fractions, and investigated the distribution of the newly synthesized RNA among the subcellular fractions isolated from first and third trimester placentas.The poly A(-) RNA was the major fraction of the RNA synthesized in vitro. The incorporation of [3H]uridine into the poly A(+) RNA fraction was very low.As protein synthesis occurred during the entire incubation period, we suggest the presence of a pool of mRNA molecules in the form of mRNP particles. |
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