8-Oxoguanine-DNA glycosylase 1 deficiency modifies allergic airway inflammation by regulating STAT6 and IL-4 in cells and in mice |
| |
Authors: | Li Guoping Yuan Kefei Yan Chunguang Fox John Gaid Madeleine Breitwieser Wayne Bansal Arvind K Zeng Huawei Gao Hongwei Wu Min |
| |
Affiliation: | Department of Biochemistry and Molecular Biology, University of North Dakota, Grand Forks, ND 58203-9037, USA. |
| |
Abstract: | 8-Oxoguanine-DNA glycosylase (OGG-1) is a base excision DNA repair enzyme; however, its function in modulating allergic diseases remains undefined. Using OGG-1 knockout (KO) mice, we show that this protein affects allergic airway inflammation after sensitization and challenge by ovalbumin(OVA). OGG-1 KO mice exhibited less inflammatory cell infiltration and reduced oxidative stress in the lungs after OVA challenge compared to WT mice. The KO phenotype included decreased IL-4, IL-6, IL-10, and IL-17 in lung tissues. In addition, OGG-1 KO mice showed decreased expression and phosphorylation of STAT6 as well as NF-κB. Down-regulation of OGG-1 by siRNA lowered ROS and IL-4 levels but increased IFN-γ production in cultured epithelial cells after exposure to house dust mite extracts. OGG-1 may affect the levels of oxidative stress and proinflammatory cytokines during asthmatic conditions. OGG-1 deficiency negatively regulates allergen-induced airway inflammatory response. |
| |
Keywords: | |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|