Effectiveness of a simplified method for isolation of Burkholderia pseudomallei from soil |
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Authors: | Limmathurotsakul Direk Wuthiekanun Vanaporn Amornchai Premjit Wongsuwan Gumphol Day Nicholas P J Peacock Sharon J |
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Institution: | Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. direk@tropmedres.ac |
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Abstract: | Detection of environmental Burkholderia pseudomallei indicates a risk for melioidosis and is important for the development of a global risk map. We describe a simple method for detecting B. pseudomallei using direct culture of soil in enrichment broth. This gives a rate of positivity comparable to that obtained with a standard method but is cheaper and labor saving. |
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