An improved rapid method for the preparation of D-rhamnose |
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| Authors: | Smol'kina Olga N Shishonkova Natalya S Fedonenko Yulia P Zdorovenko Evelina L Konnova Svetlana A Ignatov Vladimir V |
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| Institution: | Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov 410049, Russia. room308@ibppm.sgu.ru |
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| Abstract: | A method is developed for the preparation of D-rhamnose from an O-polysaccharide (OPS) isolated by mild acid hydrolysis of Azospirillum brasilense SR75 cell mass. After the OPS hydrolysis, D-rhamnose was recovered by gel-permeation chromatography on Toyopearl TSK HW-40 and was crystallized. The sugar activity was demonstrated immunochemically. The advantages of the method are that it expedites and simplifies the extraction of D-rhamnose and increases its yield. |
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