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Purification to apparent homogeneity of a thymocyte specific growth factor from calf thymus |
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| Authors: | Olof Söder Ulf Ernström |
| Affiliation: | Department of Histology, Karolinska Institutet, Stockholm, Sweden |
| Abstract: | A thymic peptide previously found to recruit thymocytes from G1 into S phase has been purified from a crude thymic extract by subsequent steps of gel exclusion chromatography and reverse phase high performance liquid chromatography (HPLC). The purified material, which appeared homogeneous on thin-layer chromatography and HPLC, stimulated the DNA synthesis of cultured guinea pig thymocytes in a nanomolar concentration range. The amino acid composition revealed a high content of acidic amino acids and no apparent homology to previously defined growth factors and thymus differentiation hormones. |
| Keywords: | TLC thin-layer chromatography HPLC high performance liquid chromatography TFA trifluoroacetic acid α-MEM α-modified Eagles Minimum Essential Medium molecular ratio pI isoelectric point TNBS trinitrobenzenesulfonic acid FTS Facteur Thymique Serique absorbance at 280 nm absorbance at 214 nm |
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