Ecdysteroid titers and changes in chromosomal activity in the salivary glands of Rhynchosciara americana |
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| Authors: | Ann Jacob Stocker Manuel Troyano-Pueyo Sirlei D Pereira F J S Lara |
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| Institution: | (1) Department of Biochemistry, University of São Paulo, São Paulo, Brazil;(2) Department of Genetics and Human Variation, La Trobe University, Bundoora, Victoria, Australia |
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| Abstract: | Titers of ecdysone and 20-OH ecdysone were measured separately in both hemolymph and salivary glands of metamorphosing Rhynchosciara larvae. Gland titers were consistently higher than hemolymph titers. Although 20-OH ecdysone was the most prominent form of the hormone, measurable quantities of ecdysone were also observed throughout development in both tissues. Changes in salivary gland replication and puffing activity could be correlated with changes in gland 20-OH ecdysone titers. This was true for both developmentally changing RNA puffs and  DNA puffs, which occur during the prepupal period. The DNA puffs are tied to the final DNA replication cycle, and both this cycle and the period of amplification can be correlated with increases in gland 20-OH ecdysone content. Various aspects and possible interpretations of the above correlations are discussed.This work is dedicated to the memory of Prof. Hans D. Berendes |
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