Microtubule organization in the apical cell of Sphacelaria (Phaeophyceae) and its related protoplast |
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Authors: | Rusig A M Le Guyader H Ducreux G |
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Institution: | (1) Morphogénèse végétale expérimentale, URA CNRS 115, Université Paris-Sud, 91405 Orsay Cedex, France;(2) Biologie cellulaire, URA CNRS 1134, Université Paris-Sud, 91405 Orsay Cedex, France |
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Abstract: | The growth of the filamentous brown alga Sphacelaria depends on a large, strongly polarized, apical cell. The protoplast derived from this cell can be distinguished in a heterogeneous suspension by cytological markers, so it is possible to study development of the cytoskeleton during protoplast isolation and the first steps of regeneration. In the initial cell, microtubules show an asymmetric distribution along the axis; they are mainly located at the distal part around the physodes. After protoplast isolation, this polarity initially seems to be maintained; subsequently, the microtubules radiate from the two centrioles and spread out to the plasmalemma. This experimental model is suitable for investigating the development of the polarity of the initial cell, and the sequence of the first morphogenetic events leading to protoplast regeneration. |
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Keywords: | Phaeophyceae protoplasts apical cells immunofluorescence microtubules cell cycle polarity |
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