Patch clamp characterization of a non-selective cation channel of ER membranes purified from Beta vulgaris taproots期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Patch clamp characterization of a non-selective cation channel of ER membranes purified from Beta vulgaris taproots

Authors:	Brito-Argáez Ligia Canto-Canché Blondy Hernández-Sotomayor Soledad Maria Teresa Martínez-Estevez Manuel Pottosin Igor I

Institution:	Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43, No 130, Chuburnáde Hidalgo, Mérida 97200, Yucatán, México; Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Calle 43, No 130, Chuburnáde Hidalgo, Mérida 97200, Yucatán, México; Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, 28045 Colima, Colima, México

Abstract:	The ER fraction from red beet taproot was purified on sucrose gradient and giant liposomes, suitable for patch clamping, were formed by dehydration–rehydration of the lipid film. Single-channel recordings on excised and attached patches revealed a large conductance (165 pS) cation (P_Cl−/P_K+ < 0.03) channel with equal conductance and relative permeability for Na⁺ and K⁺. This non-selective cation channel was also highly permeable for Ca²⁺. We failed to detect any single-channel currents activated by a direct application of d - myo -inositol 1,4,5 trisphosphate, despite the fact that the ER membranes were native.

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