Molecular evolution of the Metazoan protein kinase C multigene family |
| |
Authors: | Michael Kruse Vera Gamulin Helena Cetkovic Zeev Pancer Isabel M Müller Werner E G Müller |
| |
Institution: | 1. Abteilung Angewandte Molekularbiologie, Institut für Physiologische Chemie, Universit?t, Duesbergweg 6, 55099, Mainz, Germany 2. Department of Molecular Genetics, Institute Ruder Boskovic, 10000, Zagreb, Croatia
|
| |
Abstract: | Protein kinases C (PKCs) comprise closely related Ser/Thr kinases, ubiquitously present in animal tissues; they respond to
second messengers, e.g., Ca2+ and/or diacylglycerol, to express their activities. Two PKCs have been sequenced from Geodia cydonium, a member of the lowest multicellular animals, the sponges (Porifera). One sponge G. cydonium PKC, GCPKC1, belongs to the ``novel' (Ca2+-independent) PKC (nPKC) subfamily while the second one, GCPKC2, has the hallmarks of the ``conventional' (Ca2+-dependent) PKC (cPKC) subfamily. The alignment of the Ser/Thr catalytic kinase domains, of the predicted aa sequences for
these cDNAs with respective segments from previously reported sequences, revealed highest homology to PKCs from animals but
also distant relationships to Ser/Thr kinases from protozoa, plants, and bacteria. However, a comparison of the complete structures
of the sponge PKCs, which are—already—identical to those of nPKCs and cPKCs from higher metazoa, with the structures of protozoan,
plant, and bacterial Ser/Thr kinases indicates that the metazoan PKCs have to be distinguished from the nonmetazoan enzymes.
These data indicate that metazoan PKCs have a universal common ancestor which they share with the nonmetazoan Ser/Thr kinases
with respect to the kinase domain, but they differ from them in overall structural composition.
Received: 10 January 1996 / Accepted: 12 March 1996 |
| |
Keywords: | : Sponges — Geodia cydonium— Serine/threonine kinases — Phylogeny — Molecular systematics — Molecular evolution |
本文献已被 SpringerLink 等数据库收录! |
|