Effects of thiamine and pyridoxine on respiratory activity in Saccharomyces carlsbergensis strain 4228 |
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| Authors: | Ichiro Nakamura Naohiko Isobe Teijiro Kamihara Saburo Fukui |
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| Affiliation: | (1) Laboratory of Industrial Biochemistry, Department of Industrial Chemistry, Faculty of Engineering, Kyoto University, Kyoto, Japan;(2) Present address: Research Department, Pesticides Division, Sumitomo Chemical Co. Ltd., Takarazuka, Hyogo, Japan |
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| Abstract: | Studies were made to elucidate the relationship among the thiamine-induced growth inhibition, decrease in cellular vitamin B6 content and respiratory deficiency in Saccharomyces carlsbergensis strain 4228 [Nakamura et al., Biochem. Biophys. Res. Commun. 59, 771–776 (1974)]. Addition of pyridoxine to the thiamine-added culture at the beginning or in the course of cultivation brought about appearance of cytochrome spectra and the increase in the activity of heme-containing enzymes and in respiratory activity (QO2). The effects of pyridoxine occurred prior to the restoration of growth. Pyridoxine was effective even in the presence of high levels of glucose in the growth medium (not less than 3%). On the basis of these results, the mechanism of the effects of thiamine and pyridoxine was discussed. |
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| Keywords: | Thiamine Pyridoxine Vitamin B6 Respiratory activity Respiratory enzymes Hemecontaining enzymes Saccharomyces carlsbergensis |
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