Immunological comparison of microbial TPP-dependent non-oxidative α-keto acid decarboxylases |
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Authors: | Margaret M. Barrowman William Harnett Alan J. Scott Charles A. Fewson John R. Kusel |
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Affiliation: | Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ, U.K. |
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Abstract: | Abstract Antigenic, and hence possible evolutionary, relationships amongst various TPP-dependent non-oxidative α-keto acid decarboxylases were determined by the Ouchterlony double diffusion method and by measuring the degree of antibody-induced enzyme inhibition. The results show that: (a) phenylglyoxylate decarboxylases of various wild-type strains of Acinetobacter calcoaceticus are antigenically indistinguishable; (b) there seems to be no antigenic cross-reactivity between the phenylglyoxylate decarboxylase of A. calcoaceticus and of Pseudomonas aeruginosa or Pseudomonas putida ; and (c) no antigenic homology can be detected amongst phenylglyoxylate decarboxylase and phenylpyruvate decarboxylase of A. calcoaceticus and pyruvate decarboxylase of brewers' yeast. |
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Keywords: | Acinetobacter phenylglyoxylate decarboxylase phenylpyruvate decarboxylase Pseudomonas pyruvate decarboxylase yeast |
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