Studies of oligoadenylate formation on a poly(U) template |
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Authors: | R. Lohrmann L. E. Orgel |
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Affiliation: | (1) The Salk Institute for Biological Studies, 92112 San Diego, California, USA |
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Abstract: | We have studied a variety of condensation reactions involving poly (U) as template and isomeric adenosine dinucleotides as substrates. We find that [3'-5']-linked dinucleotides such as A3pA and pA3pA are better acceptors than the corresponding [2'-5']-linked compounds, while ImpA2pA is a better donor than ImpA3pA. The reaction between A2pA and ImpA3pA, for example, yields only 4% of product while the reaction of A3pA with ImpA2pA yields 86% of product. The more efficient condensation reactions of dimers are about as efficient as the self-condensation of ImpA. They yield a few percent of material in which five or more substrate molecules are linked together. The percentage of the natural [3'-5']-linkage in the product varies greatly, from as little as 1% to as much as 45%. |
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Keywords: | Oligoadenylates Phosphorimidazolides Template-directed polycondensation Isomer ratios Prebiotic |
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