Structure determination of the disialylated poly-(N-acetyllactosamine)-containingO-linked carbohydrate chains of equine chorionic gonadotropin |
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| Authors: | Cornelis H. Hokke Marc J. H. Roosenboom Jane E. Thomas-Oates Johannis P. Kamerling Johannes F. G. Vliegenthart |
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| Affiliation: | 1. Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, PO Box 80.075, NL-3508 TB, Utrecht, The Netherlands
2. Department of Mass Spectrometry, Utrecht University, PO Box 80.083, NL-3508 TB, Utrecht, The Netherlands
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| Abstract: | The disialylated poly-(N-acetyllactosamine)-containingO-linked oligosaccharide alditols, released by alkaline borohydride treatment of the enzymicallyN-deglycosylated β-subunit of equine chorionic chonadotropin, were purified by fast protein liquid chromatography (FPLC) on Mono Q and analysed by fast ion bombardment mass spectrometry (FAB-MS) and1H-NMR spectroscopy. The identified oligosaccharide alditols have the following structure: $$begin{gathered} Neu5Acalpha 2 - 3left[ {Galbeta 1 - 4GlcNAcbeta 1 - 3} right]_{0 - 4} Galbeta 1 - 4GlcNAcbeta 1 - 6 hfill begin{array}{*{20}c} { backslash } { GalNAc - ol} { /} {Neu5Acalpha 2 - 3Galbeta 1 - 3} end{array} hfill end{gathered}$$ |
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