In vitro selection by using mutated GCN4-bZIP peptides for analysis of peptide-DNA interactions. |
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| Authors: | H Furusawa T Morii Y Okahata |
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| Institution: | Department of Biomolecular Engineering, Tokyo Institute of Technology, Nagatsuda, Midori-ku, Yokohama, Kanagawa 226-8501, Japan. |
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| Abstract: | In vitro selection has been used as a method to determine the optimal binding site for DNA-binding proteins. We report here in vitro selection of dsDNA sequences that bind to mutated-GCN4-bZIP peptides. The GCN4-bZIP peptide mutated from alanine to histidine on a position-14 that contacts with DNA bound to different sequence from a binding site of wild type peptide. |
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