Strain improvement ofArthrobacter simplex by protoplast fusion |
| |
Authors: | Dr W-H Liu L-W Chow C-K Lo |
| |
Institution: | (1) Graduate Institute of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China;(2) Present address: Department of Food Health, Chia-Nan College of Pharmacy, Tainan, Taiwan, Republic of China |
| |
Abstract: | Anl-tryptophan auxotroph and milky mutants were derived from an inducible cholesterol oxidase-producing bacterium,Arthrobacter simplex USA18, via UV-mutagenesis. Protoplasts of these mutants and a constitutive cholesterol oxidase producer, strain US3011, were prepared by growing cells in the presence of ampicillin (20g ml–1) followed by digestion with lysozyme. Protoplast fusion between tested strains with complementary characteristics was achieved in the presence of 20–40% polyethylene glycol 6000. The fusion frequency was about 1.5–1.7×10–3. The cholesterol oxidase activity of four fusants in a cholesterol-containing medium was 20–60% higher than that of parental strains. This study demonstrated that protoplast fusion is applicable to strain improvement ofArthrobacter strains for enzyme production. |
| |
Keywords: | protoplast fusion protoplasting cholesterol oxidase Arthrobacter simplex |
本文献已被 SpringerLink 等数据库收录! |
|