Effects of hyperventilation on phosphocreatine kinetics and muscle deoxygenation during moderate-intensity plantar flexion exercise.

The effects of controlled voluntary hyperventilation (Hyp) on phosphocreatine (PCr) kinetics and muscle deoxygenation were examined during moderate-intensity plantar flexion exercise. Male subjects (n = 7) performed trials consisting of 20-min rest, 6-min exercise, and 10-min recovery in control Con; end-tidal Pco(2) (Pet(CO(2))) approximately 33 mmHg] and Hyp (Pet(CO(2)) approximately 17 mmHg) conditions. Phosphorus-31 magnetic resonance and near-infrared spectroscopy were used simultaneously to monitor intramuscular acid-base status, high-energy phosphates, and muscle oxygenation. Resting intracellular hydrogen ion concentration (H(+)](i)) was lower (P < 0.05) in Hyp 90 nM (SD 3)] than Con 96 nM (SD 4)]; however, at end exercise, H(+)](i) was greater (P < 0.05) in Hyp 128 nM (SD 19)] than Con 120 nM (SD 17)]. At rest, PCr] was not different between Con 36 mM (SD 2)] and Hyp 36 mM (SD 1)]. The time constant (tau) of PCr breakdown during transition from rest to exercise was greater (P < 0.05) in Hyp 39 s (SD 22)] than Con 32 s (SD 22)], and the PCr amplitude was greater (P < 0.05) in Hyp 26% (SD 4)] than Con 22% (SD 6)]. The deoxyhemoglobin and/or deoxymyoglobin (HHb) tau was similar between Hyp 13 s (SD 8)] and Con 10 s (SD 3)]; however, the amplitude was increased (P < 0.05) in Hyp 40 arbitrary units (au) (SD 23)] compared with Con 26 au (SD 17)]. In conclusion, our results indicate that Hyp-induced hypocapnia enhanced substrate-level phosphorylation during moderate-intensity exercise. In addition, the increased amplitude of the HHb response suggests a reduced local muscle perfusion in Hyp compared with Con.