A rapid means of sex identification inSilene latifolia by use of flow cytometry |
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| Authors: | Denise E Costich Thomas R Meagher Edward J Yurkow |
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| Institution: | (1) Department of Biological Sciences, Rutgers University, 08855-1059 Piscataway, NJ, USA;(2) Department of Pharmacology and Toxicology, College of Pharmacy, Rutgers University, 08855-0789 Piscataway, NJ, USA |
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| Abstract: | Sex identification in dioecious plants using nonflowering material would have broad applications in both basic and applied
research. We present a method using flow cytometry for diagnosing the sex of the dioecious speciesSilene latifolia Poiret (Caryophyllaceae) by means of sexual differences in nuclear DNA content and base-pair composition. Males have a significantly larger genome,
attributable to the known sex-chromosome heteromorphism. Males and females also differ in the AT/GC composition, attributable
to differences in non-recombining portions of the sex chromosomes. The two measures enable assignment of individuals to sex
with a combined error rate of 9%. These results forS. latifolia indicate useful directions for future research into sex diagnostics for other dioecious species. |
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| Keywords: | nuclear DNA content AT/GC ratio sex chromosomes Silene latifolia flow cytometry dioecy |
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