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Effect of Nitrogen on Nitrate Reductase Activity in the Nodules and Leaves of Summer Moong (Vigna radiata) |
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| Authors: | SEKHON B S; KUMAR SANJEEV; DHILLON K S; SINGH RATTAN |
| Institution: | 1 Department of Chemistry, Punjab Agricultural University Ludhiana-141004, India 2 Department of Biochemistry, Punjab Agricultural University Ludhiana-141004, India 3 Department of Soils, Punjab Agricultural University Ludhiana-141004, India |
| Abstract: | The relation of the in vivo nitrate reductase (NR) activityto growth period was studied in the nodules and the leaves ofthe summer moong (Vigna radiata). The maximum NR activity wasobserved 31 days after sowing (DAS) in the leaves and 28 DASin the case of the nodules. In a pot experiment, the effectof the various nitrogen concentrations, namely 0, 3, 6, 9 and12 mg kg–1 was studied on NR activity at three growthstages. The maximum NR activity was observed at 6 mg kg–1N during the pre-flowering stage (26 DAS). Though the noduleshave higher NR activity, its expression was limited by substrateavailability. The NR activity in the leaf could be used as anindex of NR activity in the nodules. Nitrate reductase, nitrogen, nitrate, moong, Vigna radiata |
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