Development of somatic embryos from cell suspension cultures of Norway spruce (Picea abies Karst.) |
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Authors: | M P Boulay P K Gupta P Krogstrup D J Durzan |
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Institution: | (1) Department of Environmental Horticulture, University of California, 95616 Davis, CA, USA |
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Abstract: | Cell suspension cultures were established from embryonal-suspensor masses derived from mature seeds. Transfer of cell masses on to a medium containing abscisic acid stimulated development of already established individual embryos. Somatic embryos developed shoots when supported by cheese cloth in liquid medium in Petri dishes. The percentage of well-developed roots remained low even though all embryos had root meristems. We have recovered an average of 25 plantlets from an initial PCV of ca 1 g fresh wt per 10 ml. |
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