Expression of the autofluorescent protein, DsRed2, in the recombinants of the ectomycorrhizal basidiomycete, Suillus grevillei, generated by Agrobacterium-mediated transformation |
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| Authors: | Hitoshi Murata Masahide Sunagawa Takashi Yamazaki Kazuo Shishido Tomohiro Igasaki |
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| Institution: | (1) Department of Applied Microbiology and Mushroom Science, Forestry & Forest Products Research Institute, Matsunosato 1, Tsukuba 305-8687, Japan;(2) Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama Kanagawa, 226-8501, Japan;(3) Department of Molecular Cell Biology, Forestry & Forest Products Research Institute, Matsunosato 1, Tsukuba 305-8687, Japan;(4) Present address: Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency, Tsukuba 305-8505, Japan |
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| Abstract: | Recombinants were generated from the ectomycorrhizal basidiomycete, Suillus grevillei, through agroinfection using a binary vector carrying the hygromycin B resistance and the autofluorescent protein, DsRed2, markers. DsRed2 was driven by a cis-regulatory region of the glyceraldeyde-3-phosphate dehydrogenase gene (gpd) from the wood-rotting basidiomycete, Coriolus hirsutus, which contains promoters and 5′ gpd sequences with first through fourth exons and expressed for the first time in Suillus spp. The transformation system and recombinants expressing an autofluorescent protein may be useful in genetic analysis of the symbiosis. |
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| Keywords: | Agrobacterium Autofluorescent protein DsRed2 Ectomycorrhizal fungi Suillus grevillei |
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