Formation of effective channels in alginate gel for immobilization of anchorage-dependent animal cells |
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| Authors: | Yoshihito Shirai Kenji Hashimoto Shinji Irie |
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| Institution: | (1) Department of Chemical Engineering, Faculty of Engineering, Kyoto University, Sakyo-ku, 606 Kyoto, Japan;(2) Kibun Food Chemifa Co. Ltd., 3-2-5 Shinbashi, Minato-ku, 105 Tokyo, Japan |
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| Abstract: | Summary The conditions for formation of effective channels in alginate gels for growth of anchorage-dependent animal cells were examined. Many channels were formed in the gels by adding a low concentration solution of a high molecular weight polymer of alginate to a high concentration solution of divalent cations. It is recommended that an alginate with a high molecular weight and a low mannuronic acid/guluronic acid ratio be gelled by contact with strontium ions for the cultivation of immobilized anchorage-dependent cells because the gels produced have many channels and are mechanically strong. |
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