Puromycin does not inactivate the galactrosyltransferase of Golgi membranes. |
| |
| Authors: | C A Smith J T Powell K Brew |
| |
| Affiliation: | Department of Biochemistry University of Miami School of Medicine P O Box 520875 Miami, Florida 33152, USA |
| |
| Abstract: | The effects of puromycin on galactosyltransferase from four sources, rat liver and lactating sheep mammary Golgi membranes, bovine colostrum and human serum have been investigated. We do not find that the synthesis of N-acetyllactosamine is much inhibited, even in the presence of 3mM puromycin. This is in contrast to previously reported results for rat liver Golgi membranes. We interpret the low level of inhibition observed in terms of pH effects. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
