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A new efficient expression system for Bacillus and its application to production of recombinant phytase |
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| Authors: | Janne Kerovuo Niklas von Weymarn Mira Povelainen Sanna Auer Andrei Miasnikov |
| Institution: | (1) Danisco Cultor Innovation, Kantvik Research Centre, Finland;(2) Laboratory of Bioprocess Engineering, Helsinki University of Technology, Espoo, Finland;(3) Danisco Cultor Innovation, Kantvik Research Centre, Kantvik, Finland |
| Abstract: | A new expression system was developed for Bacillus subtilis.This system uses a shuttle vector (B. subtilis– Eschericia coli) carrying a phosphate starvation-inducible promoter (pst) and on a fed-batch cultivation strategy. The pst-promoter proved to be very strong and retain its tight regulation also when present on a multi-copy plasmid. The expression system developed showed promising results when applied to the production of recombinant Bacillusphytase – phytase activity at the end of cultivation reached 28.7 U ml–1. |
| Keywords: | Bacillus subtilis fed-batch cultivation phosphate-starvation phytase plasmid |
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