Chlorophyll biosynthesis by mesophyll protoplasts and plastids from etiolated oat (Avena sativa L.) leaves |
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Authors: | Jürgen Benz Rüdiger Hampp Wolfhart Rüdiger |
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Institution: | (1) Botanisches Institut der Universität München, Menzinger Straße 67, D-8000 München 19, Federal Republic of Germany;(2) Institut für Botanik, Technische Universität München, Arcisstraße 21, D-8000 München 2, Federal Republic of Germany |
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Abstract: | The uptake of 1-3H]geranylgeranyl diphosphate (GGPP) into protoplasts and intact etioplasts and the metabolic interconversion therein was studied after a 2 min pulse of white light. The chlorophyll synthetase reaction, Chlide+GGPP ChlGG, was taken as a natural probe for the etioplast compartment. This reaction yields labeled ChLGG and, by hydrogenation, labeled ChlP, when 1-3H]GGPP receives access to the etioplast stroma. It was found that penetration across the plastid envelope was rapid and that penetration across the plasma membrane of protoplasts, however, was slow. A cellular pool of soluble GGPP was detected. This pool was lost, in part, during preparation of the protoplasts and almost completely during preparation of the etioplasts. The membrane-bound phytol pool of etioplasts could not be replaced by exogenous 3H]GG. The endogenous GG and phytol pools of protoplasts, which were larger than those of etioplasts, could be replaced in part by exogenous 3H]GGPP. That part of this pool exists as soluble GGPP or as a direct precursor in the cytoplasm is discussed.Abbreviations GGPP
geranylgeranyldiphosphate
- ChlGG
geranylgeranyl chlorophyllide a
- ChlP
phytyl chlorophyllide a
- IPP
isopentenyl diphosphate
- Chlide
chlorophyllide a |
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Keywords: | Avena Chlorophyll biosynthesis Etioplasts Geranylgeranyldiphosphate Protoplasts |
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