Polo样激酶1在细胞周期及细胞周期监测点中的功能

Plk1（Polo-like kinase 1）是一类从酵母到人类都高度保守的丝氨酸/苏氨酸蛋白激酶，是真核细胞有丝分裂的重要调控因子.Plk1随有丝分裂进程定位于不同位点，调节分裂期进入、纺锤体形成和胞质分裂等过程.Plk1能够与磷酸化的停靠蛋白结合，从而在不同空间被激活以满足其在细胞周期中的不同功能.Plk1还参与G2和M期DNA损伤监测点的调节，对于DNA损伤恢复后重新进入有丝分裂期是必须的.目前，Plk1的重要功能尤其是在DNA损伤监测点中发挥的重要功能正在被广泛研究.Plk1在多种恶性肿瘤中存在过表达且与肿瘤发生密切相关，对于Plk1功能的深入研究为以Plk1为靶的肿瘤治疗提供理论依据

Functions of Polo-like Kinase 1 in Cell Cycle and Cell Cycle Checkpoint

Polo-like kinase 1 (Plk1) belongs to a family of serine/threonine kinase that are highly conserved in organisms from yeasts to humans. Polo-like kinase 1 is a key regulator of cell division in enkaryotic cells.Plk1 involves in the regulation of different processes of mitosis,including mitotic entry ,spindle formation and cytokines. Spatial and temporal coordination of Plk1 activity is needed in agreement with its abroad functions during cell division, and this is achieved through its banding to phosphorylated docking proteins with distinct subcellular localization. Moreover, Plk1 is also reported to have functions in both G2 and mitotic DNA damage checkpoints. Plk1 is necessary for mitotic entry following recovery from DNA damage. And mitotic cells with DNA damaging agents inhibits Plk1 activity through dephosphorylation of Plk1.Recent findings are uncovering the mechanisms of Plk1 regulation, especially its involvement in the DNA damage checkpoint.It has now been broadly investigated of the mechanisms of Plk1 biological fuuction.Plk1 is overexpressed in lots of human cancers ,and overexpressin of Plk1 promotes neoplastic transformation of human cells. Plk1 has been identified as a promising target for the molecular therapy of neoplasia.