Target cell heterogeneity in murine leukemia virus infection. III. Identification of susceptible Lyt 1+ and resistant Lyt 2+ T-cell subsets following in vitro infection with Friend murine leukemia virus |
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Authors: | D D Isaak R M Miceli J P Lake |
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Affiliation: | 1. Department of Microbiology and Immunology, Kirksville College of Osteopathic Medicine, Kirksville, Missouri 63501, U.S.A.;2. Department of Pathology and Laboratory Medicine, The Jewish Hospital of St. Louis, and the Department of Pathology, Washington University School of Medicine, St. Louis, Missouri 63110 U.S.A. |
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Abstract: | The susceptibility of splenic T-cell subpopulations to productive infection with Friend murine leukemia virus was determined after in vitro infection and stimulation with Con A. Con A enhanced the number of productively infected cells in unseparated spleen cells as well as in T-cell-enriched spleen cell fractions. Splenic T cells were fractionated into Lyt 1+ and Lyt 2+ subpopulations using both positive and negative selection techniques; susceptible splenic T cells were recovered in the Lyt 1+ fraction and specific cytotoxic treatment with anti-Lyt 1 antibody and complement reduced the number of infectious center-producing cells by greater than 87%. In marked contrast, Lyt 2+ splenic T cells were resistant to productive infection by Friend murine leukemia virus in vitro. |
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