Identification of beta-tubulin isoform V as an autoantigen in allergic rhinitis by a proteomic approach |
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Authors: | Nakamura Manabu Tsutsumi Kouichiro Ooka Seido Sekine Taichi Koizuka Izumi Nishioka Kusuki Kato Tomohiro |
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Affiliation: | Department of Bioregulation, Institute of Medical Science, St. Marianna University School of Medicine, Kawasaki, Kanagawa, Japan. |
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Abstract: | Autoantibodies to IgE and beta2-adrenergic receptor have been reported in patients with allergic rhinitis. To investigate whether autoimmunity in allergic rhinitis is directed to such limited molecules or directed to a wide range of self proteins, we here attempted to survey autoantigens/autoantibodies comprehensively, using proteomics. Specifically, we separated proteins extracted from peripheral blood mononuclear cells by 2-dimensional electrophoresis and then detected autoantigens by subsequent western blotting with sera from patients with allergic rhinitis. As a result, we detected multiple autoantigens, some of which were further identified by mass fingerprinting. Next, we confirmed antigenicity of one of the identified autoantigens, beta-tubulin isoform V (beta-tubV), using a recombinant protein and then measured prevalence of the anti-beta-tubV autoantibodies. As a result, 52% of the tested patients with allergic rhinitis were found to possess anti-beta-tubV autoantibodies. Our study indicates that autoimmunity is a common phenomena and beta-tubV is one of the major autoantigens in allergic rhinitis. |
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Keywords: | β‐tubulin autoantigen allergic rhinitis proteomics |
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