Manipulating the bioactivity of hydroxyapatite nano-rods structured networks: Effects on mineral coating morphology and growth kinetic |
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Authors: | Noelia L. D'Elí a,A. Noel Gravina,Juan M. Ruso,Juan A. Laiuppa,Graciela E. Santillá n,Paula V. Messina |
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Affiliation: | 1. Department of Chemistry, Universidad Nacional del Sur, INQUISUR-CONICET, (8000) Bahía Blanca, Argentina;2. Soft Matter and Molecular Biophysics Group, Department of Applied Physics, University of Santiago de Compostela, Santiago de Compostela 15782, Spain;3. Department of Biology, Biochemistry and Pharmacy, Universidad Nacional del Sur, (8000) Bahía Blanca, Argentina |
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Abstract: | BackgroundNano-hydroxyapatite particles have better bioactivity than the coarse crystals. So, they can be utilized for engineered tissue implants with improved efficiency over other materials. The development of materials with specific bioactive characteristics is still under investigation.MethodsThe surface properties of four hydroxyapatite materials templated by different micelle-polymer structured network are studied. The synergistic interaction of each block copolymer in contact with CTAB rod-like micelles results in crystalline HAp nano-rods of 25–50 nm length organized in hierarchical structures with different micro-rough characteristics.ResultsIt was observed that the material in vitro bioactivity strongly depends on the surface structure while in a minor extent on their Ca/P ratio. So, MIII and MIV materials with Skewness parameter Rsk > 2.62 favored the formation on their surfaces of net-like phase with a high growth kinetic constant; while MI and MII (Rsk ≤ 2.62) induced the appearance of spherulitic-like structures and a growth rate 1.75 times inferior. Material biocompatibility was confirmed by interaction with rat calvarial osteoblasts.ConclusionsThe different structures growth is attributed to a dissimilar matching of crystal planes in the material and the apatite layer formed. In specific synthesis conditions, a biocompatible material with a Ca/P ratio close to that for the trabecular bone and a morphology that are considered essential for bone-bonding was obtained.General significanceThe creation of implantable devices with a specific bioactive characteristic may be useful to manipulate the attachment of cells on mineral coating directly affecting the stability and life of the implant. |
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Keywords: | Hydroxyapatite Trabecular bone Bioactivity Nano-rods Sol&ndash gel method Osteoblast viability |
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