Fibronectin conformation regulates the proangiogenic capability of tumor-associated adipogenic stromal cells |
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Authors: | Alwin M.D. Wan Emily M. Chandler Maya Madhavan David W. Infanger Christopher K. Ober Delphine Gourdon George G. Malliaras Claudia Fischbach |
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Affiliation: | 1. Department of Materials Science and Engineering, Cornell University, Ithaca, NY 14853, USA;2. Department of Biomedical Engineering, Cornell University, Ithaca, NY 14853, USA;3. Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC, 880 Route de Mimet, 13541 Gardanne, France;4. Kavli Institute at Cornell for Nanoscale Science, Cornell University, Ithaca, NY 14853, USA |
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Abstract: | BackgroundChanges in fibronectin (Fn) matrix remodeling contribute to mammary tumor angiogenesis and are related to altered behavior of adipogenic stromal cells; yet, the underlying mechanisms remain unclear due in part to a lack of reductionist model systems that allow the inherent complexity of cell-derived extracellular matrices (ECMs) to be deciphered. In particular, breast cancer-associated adipogenic stromal cells not only enhance the composition, quantity, and rigidity of deposited Fn, but also partially unfold these matrices. However, the specific effect of Fn conformation on tumor angiogenesis is undefined.MethodsDecellularized matrices and a conducting polymer device consisting of poly(3,4-ethylenedioxythiophene) doped with poly(styrenesulfonate) (PEDOT:PSS) were used to examine the effect of Fn conformation on the behavior of 3T3-L1 preadipocytes. Changes in cell adhesion and proangiogenic capability were tested via cell counting and by quantification of vascular endothelial growth factor (VEGF) secretion, respectively. Integrin-blocking antibodies were utilized to examine varied integrin specificity as a potential mechanism.ResultsOur findings suggest that tumor-associated partial unfolding of Fn decreases adhesion while enhancing VEGF secretion by breast cancer-associated adipogenic precursor cells, and that altered integrin specificity may underlie these changes.Conclusions and general significanceThese results not only have important implications for our understanding of tumorigenesis, but also enhance knowledge of cell-ECM interactions that may be harnessed for other applications including advanced tissue engineering approaches. This article is part of a Special Issue entitled Organic Bioelectronics — Novel Applications in Biomedicine. |
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Keywords: | Fibronectin Tumor angiogenesis Integrins Preadipocytes Conducting polymers Bioelectronics |
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