RGD peptides grafting onto poly(ethylene terephthalate) with well controlled densities |
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Authors: | Chollet C Chanseau C Brouillaud B Durrieu M C |
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Institution: | INSERM U577, Bordeaux, F-33076, France. celine.chollet@bordeaux.inserm.fr |
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Abstract: | The aim of this study was to graft RGD peptides with well controlled densities onto poly(ethylene terephthalate) (PET) film surfaces. Biomimetic modifications were performed by means of a four-step reaction procedure: surface modification in order to create -COOH groups onto polymer surface, coupling agent grafting and finally immobilization of peptides. The originality of this work is to evaluate several grafted densities peptides. Toluidine blue and high-resolution mu-imager (using (3)H]-Lys) were used to evaluate densities. Moreover, mu-imager has exhibited the stability of peptides grafted onto the surface when treated under harsh conditions. Benefits of the as-proposed method were related to the different concentrations of peptides grafted onto the surface as well as the capacity of RGD peptide to interact with integrin receptors. |
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