Purification and characterization of a novel carbaryl hydrolase from Aspergillus niger PY168 |
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Authors: | Zhang Qing Liu Yang Liu Yu-Huan |
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Institution: | The Key Laboratory of Gene Engineering of Ministry of Education, Sun Yet-Sen University, Guangzhou 510275, PR China. |
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Abstract: | A fungus capable of using carbaryl as the sole source of carbon and energy was isolated from a soil enrichment, and characterized as Aspergillus niger and designated strain PY168. A novel carbaryl hydrolase from cell extract was purified 262-fold to apparent homogeneity with 13.6% overall recovery. It had a monomeric structure with a molecular mass of 50,000 Da and a pI of 4.6, and the enzyme activity was optimal at 45 degrees C and pH 7.5, The activities were strongly inhibited by Hg(2+), Ag+, rho-chloromercuribenzoate, iodoacetic acid, diisofluorophosphate and phenylmethylsulfonyl fluoride but not EDTA and phenanthroline. The purified enzyme hydrolyzed various N-methylcarbamate insecticides. Carbaryl is the preferred substrate. |
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Keywords: | Carbaryl Hydrolase Aspergillus niger Degradation Carbamate insecticide |
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