食物供应量对转红色荧光蛋白基因唐鱼 生存和生长的影响

选取雄性杂合子转红色荧光蛋白基因唐鱼(Tanichthys albonubes)(简称:转基因唐鱼)与雌性非转基因唐鱼交配产卵,出膜7d后,在水温(25.0 ±2.0)℃条件下,选择健康仔鱼进行饱食(食物供应量高)、半饥饿(食物供应量中)和饥俄(食物供应量低)3种处理,结果显示:(1)出膜后7～72 d的唐鱼饱食组与半...

Effects of Food Supply Level on Survival and Growth of Transgenic Tanichthys albonubes Expressing the Red Fluorescent Protein

We assessed fitness of transgenic Tanichthys albonubes to food supply level using 7-days healthy larvae from crossing heterozygous transgenic T.albonubes expressing the red fluorescent protein to non-transgenic T.albonubes.The larvae were randomly divided into three groups: satiation group(high food supply level),semi-starvation group(medium food supply level) and starvation group(low food supply level) under(25.0 ± 2.0) ℃.The results showed that:(1) the mortality rate of 72-days of larvae in satiation group was not significantly different from those in semi-starvation group(P > 0.05),but larvae in starvation group was significant difference from both satiation group and semi-starvation group(P < 0.01).It indicates that food supply level affect the mortality rate of T.albonubes.(2) The red fluorescent protein was initially expression at 30-days larvae with visual inspection.The PCR method was used to make sure that there was no recessive expression in the three treatments at 72-days larval.At this point the number of transgenic and non-transgenic T.albonubes was close to the 1 ∶1 ratio,and consistent with classical Mendelian inheritance model,which indicates that transgenic and non-transgenic T.albonubes are equally effected by food supply level.(3) The body length at 28-,42-,57-and 72-days larvae was satiation group > semi-starvation group > starvation group at significant or very significant level(P < 0.05 or P < 0.01).The larvae body length of 72-days larvae has no significantly between transgenic and non-transgenic ones within the same food supply level group.In summary,the food supply level had similar influence on survival and growth of T.albonubes for both transgenic and non-transgenic.We speculate that transgenic T.albonubes have no competition advantage in the low food availability.