Quantitative Addition of Dissolved Oxygen to In Situ Benthic Chamber Systems by Use of Catalase and Hydrogen Peroxide

A methodology for reoxygenation of in situ benthic chamber systems by enzymatic catalysis of hydrogen peroxide with catalase was developed. For a 10-liter benthic chamber, the injection of 1 ml of catalase suspension (26,000 U ml⁻¹) followed by 10 ml of 0.5 M hydrogen peroxide solution resulted in complete reoxygenation within 2.5 min at 25°C.