Tissue-specific expression of rice CYP72A21 induced by auxins and herbicides |
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Authors: | Sakiko Hirose Hiroyuki Kawahigashi Akemi Tagiri Hiromasa Imaishi Hideo Ohkawa Yasunobu Ohkawa |
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Institution: | (1) Plant Biotechnology Department, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba Ibaraki, 305-8602, Japan;(2) Research Center for Environmental Genomics, Kobe University, Rokkodaicho 1-1, Nada Kobe, 657-8501, Japan;(3) Faculty of Life Science and Biotechnology, Fukuyama University, Gakuencho 1, Fukuyama Hiroshima, 729-0292, Japan;(4) Secretariat of the Agriculture, Forestry and Fisheries Research Council, MAFF, 1-2-1 Kasumigaseki, Chiyoda-ku Tokyo, 100-8950, Japan |
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Abstract: | Cytochrome monooxygenase P450s (CYPs) comprise one of the largest enzyme families in plants. Some P450s are involved in xenobiotic
metabolism: they confer herbicide tolerance and are induced by chemical treatments. We isolated a novel P450 cDNA, CYP72A21 (accession number, AB237166), from rice (Oryza sativa L. cv. Nipponbare) seedlings treated with a mixture of 2,4-dichlorophenoxyacetic acid (2,4-D), chlorotoluron, phenobarbital,
salicylic acid, and naphthalic anhydride (each 100 μM). We also isolated the gene’s promoter region. Endogenous CYP72A21 expression in rice seedlings treated with 2,4-D, herbicides esprocarb, or trifluralin was increased in the aerial part of
seedlings. An expression plasmid, pI21pg, containing the GUS gene under the control of the CYP72A21 promoter was introduced into rice plants. GUS was expressed constitutively in roots, but this expression was suppressed by 2,4-D treatment. 2,4-D and other auxins induced
GUS expression effectively in the stem and leaves. Histological observation revealed that GUS was expressed mainly in the base of the stem. Treatment with the herbicides acetochlor, esprocarb, and propyzamide induced
GUS expression in the aerial parts of the seedlings. The CYP72A21 promoter was highly responsive to treatments with various chemicals, and thus might be useful for producing transgenic plants
for biomonitoring of environmental chemicals. |
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Keywords: | 2 4-D Acetochlor Cytochrome P450 Esprocarb Propyzamide Trifluralin |
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