一种简单快速高分辨率的PAGE胶显带方法

尽管使用常规方法对聚丙烯酰胺胶(PAGE胶)进行DNA显带, 能获得高分辨率图片, 但常规方法操作步骤繁琐, 耗时较长。文章报道了一种PAGE胶DNA显带的改进方法, 分别使用改进的显带方法和常规的显带方法进行了PAGE胶的显影和比较。结果表明, 使用改进的显带方法得到的PAGE胶图片, DNA带型和背景具有更高的对比度, 因此具有更高的分辨率; 同时操作步骤少, 耗时短, 使用试剂少。这种方法在本实验室中已经完全代替了常规PAGE胶显带方法。

A simple, rapid and high-resolution banding method in polyacryla-mide gels

Although high-resolution picture can be obtained using the conventional banding method of DNA in PAGE gel, this method has complicated steps and will take very long time. In this paper, we reported an improved banding method of DNA in PAGE gel. DNA development in PAGE gel and comparison were performed by using two methods, the conventional banding method and the improved banding method, respectively. And the results indicated that the improved method has higher contrast between DNA band and the background in PAGE gel. So this improved method has higher resolution than the conventional one. Compared with the conventional banding method, this improved method has fewer steps, less time took and fewer reagents used. This improved method has replaced the conventional banding method completely in our laboratory.