过表达长非编码RNA SLC25A25-AS1可抑制HeLa细胞的增殖、迁移和侵袭

长非编码RNA SLC25A25-AS1在结直肠癌的发展中具有肿瘤抑制作用，然而，其在宫颈癌中作用机制有待深入研究。本文研究了宫颈癌和宫颈上皮内瘤变（cervical intraepithelial neoplasia,CIN）病人血清中SLC25A25-AS1的异常表达，并探讨了SLC25A25-AS1在宫颈癌发展中可能的作用机制。采用RT-qPCR检测正常对照组，宫颈癌病人和CIN病人血清中SLC25A25-AS1的表达水平。在体外和体内研究中分析了SLC25A25-AS1在宫颈癌系HeLa细胞中的重要作用。与健康对照组相比，宫颈癌病人血清中的SLC25A25-AS1含量降低。在体外，SLC25A25-AS1的过表达抑制HeLa细胞的增殖、迁移和侵袭。体内裸鼠成瘤实验表明，注射过SLC25A25-AS1过表达的HeLa细胞的裸鼠皮下肿瘤的重量和体积明显小于注射对照细胞的裸鼠（P<0.05）。SLC25A25-AS1在宫颈癌的发生发展中可能发挥重要作用，有望成为宫颈癌的新治疗靶点。

Overexpression of LncRNA SLC25A25-AS1 Inhibits Proliferation,Migration, and Invasion of HeLa Cells

Long non-coding RNA SLC25A25-AS1 has a tumor inhibition effect in the development of colorectal cancer. However, the mechanism of SLC25A25-AS1 in cervical cancer needs further study. We studied the abnormal expression of SLC25A25-AS1 in the serum of the patients with cervical cancer and the patients with cervical intraepithelial neoplasia (CIN) and explored the mechanism of SLC25A25-AS1 in the development of cervical cancer. The expression levels of SLC25A25-AS1 in the serum of normal controls, patients with cervical cancer, and patients with CIN were detected by RT-qPCR. The important role of SLC25A25-AS1 in HeLa cells was analyzed in vitro and in vivo experiments. Compared with the normal group, the expression level of serum SLC25A25-AS1 was decreased in patients with cervical cancer. In vitro, overexpression of SLC25A25-AS1 inhibited cell proliferation, migration, and invasion of HeLa cells. Tumor formation in nude mice assay showed that the subcutaneous tumor weight and volume of nude mice injected with SLC25A25-AS1-overexpressed HeLa cells were significantly smaller than that of nude mice injected with control cells (P<0.05). SLC25A25-AS1 may play an important role in the development of cervical cancer and may serve as a new therapeutic target for cervical cancer.