Application of ultra-performance LC-TOF MS metabolite profiling techniques to the analysis of medicinal <Emphasis Type="Italic">Panax</Emphasis> herbs |
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Authors: | Guo X Xie Yan Ni Ming M Su Yuan Y Zhang Ai H Zhao Xian F Gao Zhong Liu Pei G Xiao Wei Jia |
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Institution: | (1) Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China;(2) School of Pharmacy, Shanghai Jiao Tong University, Shanghai , 200240, People’s Republic of China;(3) Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100094, China |
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Abstract: | The morphological appearance and some ingredients of Panax ginseng, Panax notoginseng and Panax
japonicus of the Panax genus are similar. However, their pharmacological activities are obviously different due to the significant differences in
the types and quantity of saponins in each herb. In the present study, ultra-performance liquid chromatography-quadrupole
time-of-flight mass spectrometry (UPLC-QTOFMS) was used to profile the abundances of metabolites in the three medicinal Panax herbs. Multivariate statistical analysis technique, that is, principle component analysis (PCA) and partial least squares-discriminant
analysis (PLS-DA) were used to discriminate between the Panax samples. PCA of the analytical data showed a clear separation of compositions among the three medicinal herbs. The critical markers such as chikusetsusaponin IVa, ginsenoside R0, ginsenoside Rc, ginsenoside Rb1, ginsenoside Rb2 and
ginsenoside Rg2 accountable for such variations were identified through the corresponding loading weights, and the tentative
identification of biomarkers is completed by the accurate mass of TOFMS and high resolution and high retention time reproducibility
performed by UPLC. The proposed analytical method coupled with multivariate statistical analysis is reliable to analyze a
group of metabolites present in the herbal extracts and other natural products. This method can be further utilized to evaluate
chemical components obtained from different plants and/or the plants of different geographical locations, thereby classifying
the medicinal plant resources and potentially elucidating the mechanism of inherent phytochemical diversity. |
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Keywords: | Panax Metabolite profiling UPLC-QTOFMS Saponin Multivariate statistical analysis |
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