Nucleotide excision repair: DNA damage recognition and preincision complex assembly |
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Authors: | N I Rechkunova Yu S Krasikova O I Lavrik |
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Institution: | Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia. nadyarec@niboch.nsc.ru |
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Abstract: | Nucleotide excision repair (NER) is one of the major DNA repair pathways in eukaryotic cells counteracting genetic changes
caused by DNA damage. NER removes a wide set of structurally diverse lesions such as pyrimidine dimers arising upon UV irradiation
and bulky chemical adducts arising upon exposure to carcinogens or chemotherapeutic drugs. NER defects lead to severe diseases
including some forms of cancer. In view of the broad substrate specificity of NER, it is of interest to understand how a certain
set of proteins recognizes various DNA lesions in the context of a large excess of intact DNA. This review focuses on DNA
damage recognition and following stages resulting in preincision complex assembly, the key and still most unclear steps of
NER. The major models of primary damage recognition and preincision complex assembly are considered. The contribution of affinity
labeling techniques in study of this process is discussed. |
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