卵巢癌组织中UHRF1蛋白的表达及其与卵巢癌细胞增殖和侵袭的关系

目的:研究卵巢癌组织中泛素样含PHD和环指域1(Ubiquitin-like with PHD and ring finger domains 1,UHRF1)蛋白的表达及UHRF1对卵巢癌细胞增殖、侵袭的影响。方法:选取卵巢癌组织和癌旁正常组织,采用蛋白印迹法(Western blot)检测其UHRF1的蛋白表达。体外培养卵巢癌SKOV-3细胞株,分别转染UHRF1的si RNA和阴性对照si RNA,采用CCK-8检测细胞活力,Transwell检测细胞侵袭能力,荧光定量聚合酶链式反应法(FQ-PCR)检测Cyclin D1、CDK6、MMP2和MMP9的m RNA表达。结果:卵巢癌组织中UHRF1蛋白表达水平显著高于癌旁正常组织(P0.05);与转染阴性对照si RNA的SKOV-3细胞相比,转染UHRF1的si RNA的SKOV-3细胞活力明显降低、侵袭细胞数目明显减少(P0.05),且细胞中Cyclin D1、CDK6、MMP2和MMP9基因的m RNA表达水平显著降低(P0.05)。结论:UHRF1蛋白在卵巢癌组织中呈高表达状态,且可促进卵巢癌细胞的增殖和侵袭。

Expression of UHRF1 Protein in Ovarian Cancer Tissue and Its Correlation with the Proliferation and Invasion of Ovarian Cancer Cells

ABSTRACT Objective: To study the expression of ubiquitin-like with PHD and ring finger domains 1(UHRF1) protein in ovarian cancer tissue and the effect of UHRF1 on the proliferation and invasion of ovarian cancer cells. Methods: Ovarian cancer tissue and adja- cent normal tissue were collected, the expresion of UHRF1 protein were detected by Westerrn blot. Ovarian cancer SKOV-3 cell was cul- tured in vitro, UHRF1 siRNA and negative control siRNA were transfected. Then the cell viability was assayed by CCK-8 kit, cell inva- sion capability was assayed by Transwell. Expressions of CyclinD1, CDK6, MMP2 and MMP9 mRNA were assayed by FQ-PCR. Results: The expression of UHRF1 protein in ovarian cancer tissue was higher than that in adjacent normal tissue. The cell viability of SKOV-3 cells and number of migrated cells transfected by UHRF1siRNA was significantly lower than that of SKOV-3 cell transfected by negative control siRNA, the expressions of CyclinD1, CDK6, MMP2 and MMP9 mRNA were significantly decreased. Conclusion: The expression of UHRF1 protein was significantly increased in ovarian cancer; UHRF1 could promote the proliferation and invasion of ovarian cancer cell.