microRNA-21对人舌鳞癌细胞的增殖和凋亡的影响

目的:探讨micro RNA-21(mi R-21)对人舌鳞癌细胞增殖和凋亡的影响。方法:选取8例舌鳞癌组织和4例癌旁组织为研究材料,采用实时荧光定量聚合酶链式反应(q RT-PCR)法对舌鳞癌及癌旁组织中的mi R-21相对表达量进行检测,利用人工合成的mi R-21mimic对人舌鳞癌Tca8113细胞进行瞬时转染,采用q RT-PCR法对Tca8113细胞中mi R-21相对表达量进行检测,采用四唑盐比色法(MTT)法对Tca8113细胞增殖情况进行检测,采用流式细胞术对Tca8113细胞周期与凋亡情况进行检测。结果:舌鳞癌组织中mi R-21的相对表达量(3.502±0.674),高于癌旁组织(0.998±0.192),差异有统计学意义(P0.05)。mi R-21mimic导致了Tca8113细胞中的mi R-21相对表达量上调(6.864±1.324),明显高于对照scramble组(0.997±0.187),P0.05],对Tca8113细胞的增殖发挥了促进作用(P0.05)。经mi R-21mimic转染之后,Tca8113细胞进入S期的细胞出现了明显的增加(27.4±5.1)%vs(48.6±8.7)%,P0.05],处于G1期的细胞出现了显著的减少(56.3±9.6)%vs(36.2±7.2)%,P0.05],细胞凋亡数量出现了显著减少(9.4±2.3)%vs(18.6±3.9)%,P0.05]。结论:mi R-21在舌鳞癌组织中高表达,过表达mi R-21有效促进了Tca8113细胞的增殖,抑制细胞的凋亡,mi R-21在舌鳞癌诊断和治疗可能具有一定的新型靶点价值。

Effect of microRNA-21 on Proliferation and Apoptosis of Human Tongue Squamous Carcinoma Cell

ABSTRACT Objective: The aim of this study is to investigate the effect of microRNA-21(miR-21) on proliferation and apoptosis of human tongue squamous carcinoma cell. Methods: The miR-21 expression was detected in 8 tongue squamous cell carcinoma tissues and 4 adjacent tissues by Real-time quantitative PCR (qRT-PCR). Artificially synthesized miR-21 mimic was transiently transfected into hu- man tongue squamous cell carcinoma Tca8113 cell, and then the expression of miR-21 was tested by qRT-PCR. MTT assay was used to detect the cell proliferation of Tca8113 cell, apoptosis and cell cycle of Tca8113 cell were measured by flow cytometr. Results: The miR-21 expression of tongue squamous carcinoma tissues was higher than that in adjacent tissues (3.502±0.674) vs (0.998±0.192), (P<0.05)]. The transient transfection of miR-21 mimic into Tca8113 cell significantly increased the miR-21expression of Tca8113 cell(6.864±1.324) vs(0.997±0.187), P<0.05], promoted cell proliferation, raised the cell proportion in S phase( 27.4±5.1)% vs (48.6±8.7)%, P<0.05], reduced the cell proportion in G1 phase (56.3±9.6)% vs (36.2±7.2)%, P<0.05], and inhibited cell apoptosis(9.4±2.3)% vs (18.6±3.9)%, P<0.05]. Conclusion: miR-21 was over-expressed in tongue squamous carcinoma tissues. Transient transfections of miR-21 mimic lead to increased proliferation and reduced apoptosis in Tca8113 cell, which suggested that miR-21 maybe a target for the treatment of tongue squamous carcinoma.